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KMID : 0604520150410040361
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Journal of the Society of Cosmetic Scientists of Korea
2015 Volume.41 No. 4 p.361 ~ p.373
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Antioxidant and Antibacterial Activities of Glycyrrhiza uralensis Fisher (Jecheon, Korea) Extracts Obtained by various Extract Conditi
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Ha Ji-Hoon
Jeong Yoon-Ju
Seong Joon-Seob
Kim Kyoung-Mi
Kim A-Young
Fu Min-Min
Suh Ji-Young
Lee Nan-Hee
Park Jin-Oh
Park Soo-Nam
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Abstract
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This study was carried out to evaluate the antioxidant and antibacterial activities of Glycyrriza uralensis Fisher (Jecheon, Korea) extracts obtained by various extraction conditions (85% ethanol, heating temperatures and times), and to establish the optimal extraction condition of G. uralensis for the application as cosmetic ingredients. The extracts obtained under different conditions were concentrated and made in the powdered (sample-1) and were the crude extract solutions without concentration (sample-2). The antioxidant effects were determined by free radical scavenging activity (FSC50), ROS scavenging activity (OSC50), and cellular protective effects. Antibacterial activity was determined by minimum inhibitory concentration (MIC) on human skin flora. DPPH free radical scavenging activity of sample-1 (100 μg/mL) was 10% higher in group extracted for 6 h than 12 h, but sample-2 didn’t show any significant differences. The extraction yield extracted with same temperature for 12 h was 2.6 times higher than 6 h, but total flavonoid content was 1.1 times higher. These results indicated that total flavonoid content hardly increased with increasing extraction time. Free radical scavenging activity, ROS scavenging activity and cellular protective effects were not dependent on the yield of extraction, but total flavonoid content of extraction. Antibacterial activity on three skin flora (S. aureus, B. subtilis, P. acnes)of sample-1 in different extraction conditions were evaluated on same concentration, and the group extracted at 25 and 40 ℃ showed 16 times higher than methyl paraben (2,500 μg/mL). In conclusion, 85% ethanol extracts of G. uralensis extracted at 40 ℃ for 6 h showed the highest antioxidant and antibacterial activity. These results indicate that the extraction condition is important to be optimized by comprehensive evaluation of extraction yield with various conditions, yield of active component, and activity test with concentrations, and activity of 100% extract, for manufacturing process of products.
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KEYWORD
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Glycyrrhiza uralensis, antioxidative effect, antibacterial activity, various extraction conditions, cellular protective effect
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